H9903
1-(Heptafluorobutyryl)imidazole
BioReagent, suitable for derivatization
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1-(Perfluorobutyryl)imidazole
C7H3F7N2O
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product line
BioReagent
Quality Level
bp
158-163 °C (lit.)
suitability
suitable for derivatization
storage temp.
−20°C
SMILES string
FC(F)(F)C(F)(F)C(F)(F)C(=O)n1ccnc1
InChI
1S/C7H3F7N2O/c8-5(9,6(10,11)7(12,13)14)4(17)16-2-1-15-3-16/h1-3H
InChI key
MSYHGYDAVLDKCE-UHFFFAOYSA-N
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General description
1-(Heptafluorobutyryl)imidazole, also known as HFBI, is an acylating derivatization reagent. It is a mild amine-group derivatizing agent. HFBI doesn’t produce acidic by-products in reaction, and the unutilized reagent doesn’t damage the chromatographic process. Post derivatization reaction, the HFB (Heptafluorobutyryl) derivatives are analyzed chromatographically.
Application
1-(Heptafluorobutyryl)imidazole is used as a derivatizing agent for analysing Sulphur mustard metabolites. It is suitable to study dispersive derivatization of degradation products.
Biochem/physiol Actions
Mild amine-group derivatizing reagent; non-acidic by-product prevents decomposition and reduces GC column degradation.
WGK
WGK 3
Flash Point(F)
170.6 °F
Flash Point(C)
77 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Journal of chromatography. A, 1218(32), 5393-5400 (2011-07-09)
A new derivatization and extraction technique termed as dispersive derivatization liquid-liquid extraction (DDLLE) speeds up the analysis process by removing the requirement for drying of the sample. The derivatization process takes place at the interface between the analyte containing aqueous
Archives of toxicology, 74(4-5), 207-214 (2000-08-26)
The development of a procedure for retrospective detection and quantitation of exposure to the arsenical dichloro(2-chlorovinyl)arsine (lewisite; L1) has been initiated. Upon incubation of human blood with [14C]L1 (20 nM-0.2 mM) in vitro, more than 90% of the total radioactivity
Gas chromatographic determination of 2-ethylhexanol and 2-ethylhexanoic acid as derivatives suitable for electron-capture and nitrogen-phosphorus detection after single reaction with heptafluorobutyrylimidazole.
Journal of chromatography, 509(2), 383-389 (1990-06-22)
Methods in molecular biology (Clifton, N.J.), 824, 527-534 (2011-12-14)
Two main hurdles hinder the widespread acceptance of plants as a preferred protein expression platform: low accumulation levels and expensive chromatographic purification methods. Fusion of proteins of interest to fungal hydrophobins has provided a tool to address both accumulation and
Talanta, 85(2), 1154-1159 (2011-07-06)
The N-terminal valine adduct (HETE-Val) in globin is believed to behave as a long-lived biomarker after exposure to sulfur mustard (HD). Development of a highly sensitive method for monitoring HETE-Val, particularly at low HD exposure levels or for retrospective detection
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