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H9539

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02263062

Key Documents

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Product Name

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using 3T3 or PC12 cell lysates.

UniProt accession no.

P09429

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

Related Categories

Application

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence.

Biochem/physiol Actions

In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12.

Immunogen

synthetic peptide corresponding to amino acid 165-180 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000204908
0000176842
0000164121
0000164121
0000204908

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Anjan K Bongoni et al.
Transplantation direct, 5(4), e341-e341 (2019-04-18)
Complement activation plays an important role in the pathogenesis of renal ischemia-reperfusion (IR) injury (IRI), but whether this involves damage to the vasculoprotective endothelial glycocalyx is not clear. We investigated the impact of complement activation on glycocalyx integrity and renal
Tianle Sun et al.
Immunology and cell biology, 101(8), 735-745 (2023-05-31)
Coxsackievirus B3 (CVB3)-induced viral myocarditis (VMC) is characterized by immune cell infiltration and myocardial damage. High mobility group box 1 (HMGB1) is a highly conserved nuclear DNA-binding protein that participates in DNA replication, transcriptional regulation, repair response and inflammatory response
Yu Qiu et al.
Journal of cellular and molecular medicine, 25(17), 8244-8260 (2021-08-20)
Septic cardiomyopathy is a common complication of sepsis with high morbidity and mortality, but lacks specific therapy. This study aimed to reveal the role of circTLK1 and its potential mechanisms in septic cardiomyopathy. The in vitro and in vivo models
MiR-142-3p functions as a potential tumor suppressor directly targeting HMGB1 in non-small-cell lung carcinoma.
Xiao P and Liu WL
International Journal of Clinical and Experimental Pathology (2015)
Liwei Weng et al.
Analytical chemistry, 90(12), 7552-7560 (2018-05-24)
High mobility group box 1 (HMGB1) is a non-histone chromosomal protein, which can be secreted through a variety of pathways and bind to pattern recognition receptors to release pro-inflammatory cytokines. Previous studies have suggested that HMGB1 is upregulated in numerous
View All Related Papers

Related Content

Data Sheet - H9539

Instructions

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