H9536
Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Anti-HDAC4
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~140 kDa
species reactivity
human, mouse, rat
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunoprecipitation (IP): 10-20 μg using RIPA extract of HeLa nuclei
indirect immunofluorescence: 1:250 using HEK 293T cells expressing recombinant mouse HDAC4
microarray: suitable
western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells
western blot: 1:500 using whole extracts of rat brain
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
Related Categories
General description
Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position.
Mammalian histone deacetylases (HDACs) are classified into three classes according to sequence homology. HDAC1, HDAC2, HDAC3 and HDAC8 belong to class I HDACs and HDAC4, HDAC6, and HDAC7 belong to class II HDACs. Class III consists of the yeast sirtuins (sir2-like protein). Class I HDACs are ubiquitously expressed. Whereas, most of the class II HDACs show tissue-specific expression. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Specificity
Anti-Histone Deacetylase 4 (HDAC4), recognizes human, mouse and rat HDAC4.
Immunogen
Synthetic peptide corresponding to amino acid residues of human HDAC4 with C-terminal added lysine conjugated to KLH.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below:
- Western Blotting
- Immnofluorescence
- Immunoprecipitation
Biochem/physiol Actions
Histone deacetylase 4 (HDAC4) contains import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor myocyte enhancer factor-2 (Mef2) protein and 14-3-3. This interaction disturbs nucleocytoplasmic shuttling and then guides HDAC4 to the cytoplasm and the nucleus, respectively. In addition, HDAC4 with HDAC5 and HDAC7 represses transcription by binding to the nuclear receptor co-repressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (NCOR).
Studies show that HDAC4 contains a composed import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor MEF2C and 14-3-3. This interaction results in the shuttling and it guides HDAC4 to the cytoplasm and the nucleus.The C-terminal domain of HDAC4 shows innate histone deacetylase activity. It is seen that MEF2C combines with HDAC4 at the N-terminal domain and downmodulate c-jun promoter activity.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Michal Mielcarek et al.
PloS one, 6(11), e27746-e27746 (2011-12-06)
Huntington's disease (HD) is a progressive neurological disorder for which there are no disease-modifying treatments. Transcriptional dysregulation is a major molecular feature of HD, which significantly contributes to disease progression. Therefore, the development of histone deacetylase (HDAC) inhibitors as therapeutics
Joel D Federspiel et al.
Molecular & cellular proteomics : MCP, 18(8 suppl 1), S92-S113 (2019-05-02)
Huntington's disease (HD) is a monogenic disorder, driven by the expansion of a trinucleotide (CAG) repeat within the huntingtin (Htt) gene and culminating in neuronal degeneration in the brain, predominantly in the striatum and cortex. Histone deacetylase 4 (Hdac4) was
Carina Mello Guimaraes Meyers et al.
Bone, 159, 116393-116393 (2022-03-24)
Protein kinase D (PRKD) family kinases are required for formation and function of osteoclasts. However, the substrates of PRKD in osteoclasts are unknown. To identify PRKD-dependent protein phosphorylation in osteoclasts, we performed a quantitative LC-MS/MS phosphoproteomics screen for proteins showing
A H Wang et al.
Molecular and cellular biology, 19(11), 7816-7827 (1999-10-19)
Histone acetylation plays an important role in regulating chromatin structure and thus gene expression. Here we describe the functional characterization of HDAC4, a human histone deacetylase whose C-terminal part displays significant sequence similarity to the deacetylase domain of yeast HDA1.
Hdac4 interactions in Huntington's Disease viewed through the prism of multiomics
<BIG>Federspiel JD, et al.</BIG>
Molecular and Cellular Proteomics (2019)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service