H9394
Hanks′ Balanced Salt solution
Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
HBSS
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L
shipped in
ambient
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General description
Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.
Application
Hanks′ Balanced Salt solution has been used:
- as a component of the culture medium for culturing of mouse cortical slices
- as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
- for in situ perfusions of mice liver
Biochem/physiol Actions
Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
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