H9039
Hemin
from bovine, ≥90%
Synonym(s):
Chloro(protoporphyrinato)iron(III), Chlorohemin, Chloroprotoporphyrin IX iron(III), Ferriprotoporphyrin IX chloride, Hemin(chloride)
About This Item
biological source
bovine
Quality Level
Assay
≥90%
form
powder
technique(s)
toxicology assay: suitable
solubility
1.4 M NH4OH: soluble 25 mg/mL
storage temp.
2-8°C
SMILES string
Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C
InChI
1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;
InChI key
BTIJJDXEELBZFS-HXFTUNQESA-K
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General description
Application
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the solubility of Hemin, Product H9039?
Hemin can be dissolved in basic solution. A 25 mg/ml solution can be prepared in 1.4 M ammonium hydroxide with heating to 95°C for less than 5 minutes. Hemin is also expected to be soluble in a sodium hydroxide solution. Hemin has been reported to be soluble at 1 mM concentration in DMSO, per Tomlinson and Ferguson, Proceedings of the National Academy of Sciences, 97, 5156-5160 (2000). A 0.25% hemin solution can also be prepared in 50% triethanolamine solution.
How stable is Hemin, Product H9039, in solution?
Sigma-Aldrich has not evaluated the solution stability of hemin solutions. Also, we have not found any information on solution storage in the literature. We recommend that solutions be prepared fresh.
What is the shelf life for Hemin, Product H9039?
There is no shelf life date assigned to this product. The guarantee of quality is for one year from the time of shipment for bottles stored as recommended.
What are some applications for Hemin, Product H9039?
Hemin-agarose has reportedly been prepared for use in affinity chromatography of heme-binding proteins. See Tsutsui, K., Affinity chromatography of heme-binding proteins: Synthesis of hemin-agarose. Meth. Enzymol. 123, 331, (1986). Sigma supplies a hemin agarose, product H6390. Hemin can also be used as a component in culture media for the cultivation of bacteria, such as Leishmania tarentolae.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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