H8898
Halocarbon oil 700
Synonym(s):
Chlorotrifluoroethene homopolymer
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About This Item
CAS Number:
UNSPSC Code:
12162002
PubChem Substance ID:
NACRES:
NA.75
MDL number:
SMILES string
FC(=C(F)Cl)F
InChI
1S/C2ClF3/c3-1(4)2(5)6
InChI key
UUAGAQFQZIEFAH-UHFFFAOYSA-N
form
liquid
Quality Level
Application
Halocarbon oil 700 has been used:
- to facilitate piercing of vitelline membranes with a glass capillary to make small holes on spider embryos
- in microinjection procedure to track the vesicular stomatitis virus infection in Caenorhabditis elegans
- for live imaging of embryo sample using Zeiss LSM710 confocal system
General description
Halocarbon oil 700 is used for mounting worms on agarose pads. It facilitates movement of worms for microscopic view. The halocarbon acts as a source of oxygen and blocks desiccation during optical digital embryo sampling.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Lunhao Chen et al.
Frontiers in neuroscience, 12, 324-324 (2018-06-06)
Here we described an experimental protocol for in vivo imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPγS into transparent Drosophila melanogaster embryos. Using a confocal microscope for live imaging, we
C. elegans: A Practical Approach, 81-81 (1999)
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology, 514-514 (1995)
André Ferreira Castro et al.
eLife, 9 (2020-11-27)
Class I ventral posterior dendritic arborisation (c1vpda) proprioceptive sensory neurons respond to contractions in the Drosophila larval body wall during crawling. Their dendritic branches run along the direction of contraction, possibly a functional requirement to maximise membrane curvature during crawling
Amrita Ray Chaudhury et al.
Lab on a chip, 17(13), 2303-2322 (2017-06-15)
We present a microfluidic chip for immobilizing Drosophila melanogaster larvae for high resolution in vivo imaging. The chip creates a low-temperature micro-environment that anaesthetizes and immobilizes the larva in under 3 minutes. We characterized the temperature distribution within the chip
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