H8509
β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides
Type V, lyophilized powder, 250-750 units/mg protein
Synonym(s):
(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-830-6
MDL number:
Specific activity:
250-750 units/mg protein
Product Name
β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides, Type V, lyophilized powder, 250-750 units/mg protein
type
Type V
form
lyophilized powder
specific activity
250-750 units/mg protein
purified by
chromatography
foreign activity
lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%
storage temp.
−20°C
Quality Level
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Application
Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Biochem/physiol Actions
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Other Notes
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
Physical form
Lyophilized powder containing Tris buffer salts
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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C García de María et al.
Talanta, 80(3), 1436-1440 (2009-12-17)
A new kinetic method is proposed for the simultaneous determination of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) based on the different rate of the 3-hydroxybutyrate dehydrogenase-catalysed reactions of these compounds with coenzyme NAD(+). A flow injection system with two reactors of
Caiping Mao et al.
Drug and chemical toxicology, 35(2), 127-133 (2011-08-19)
To investigate whether folic acid would have toxic effects on fetal cardiac, hepatic, and renal functions, this was the first in utero fetal study testing acute effects of folic acid at the last third of gestation. Folic acid (5 mg/day) or
Takashi Ide
Bioscience, biotechnology, and biochemistry, 74(8), 1578-1582 (2010-08-12)
An enzymatic-HPLC method to analyze the serum concentration of D-3-hydroxybutyric acid was developed. A deproteinized sample of rat serum was treated with 3-hydroxybutyrate dehydrogenase in the presence of NAD, and was analyzed by reversed-phase HPLC to separate and quantify NADH
Tina Hölscher et al.
Applied and environmental microbiology, 76(16), 5585-5591 (2010-06-29)
In this study, a methylotrophic bacterium, Methylobacterium rhodesianum MB 126, was used for the production of the chiral compound (R)-3-hydroxybutyrate (R-3HB) from methanol. R-3HB is formed during intracellular degradation of the storage polymer (R)-3-polyhydroxybutyrate (PHB). Since the monomer R-3HB does
W Hugo Ramos-Vera et al.
Journal of bacteriology, 193(5), 1201-1211 (2010-12-21)
Two autotrophic carbon fixation cycles have been identified in Crenarchaeota. The dicarboxylate/4-hydroxybutyrate cycle functions in anaerobic or microaerobic autotrophic members of the Thermoproteales and Desulfurococcales. The 3-hydroxypropionate/4-hydroxybutyrate cycle occurs in aerobic autotrophic Sulfolobales; a similar cycle may operate in autotrophic
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