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Key Documents

Safety Information

H8280

Sigma-Aldrich

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

matrix fast-flow highly cross-linked 4% beaded agarose

Synonym(s):

N-Hydroxysuccinimidyl-Agarose

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5 ML
CN¥1,446.06
15 ML
CN¥3,109.05

CN¥1,446.06

Estimated to ship onJuly 21, 2025Details

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5 ML
CN¥1,446.06
15 ML
CN¥3,109.05

About This Item

MDL number:
MFCD00164950
UNSPSC Code:
23151817
NACRES:
NA.56

CN¥1,446.06

Estimated to ship onJuly 21, 2025Details

form

isopropanol suspension

Quality Level

200

extent of labeling

16-23 μmol per mL

technique(s)

protein array: suitable

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix active group

N-hydroxysuccinimide active ester

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

storage temp.

2-8°C

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1 of 4

This Item
C5338P7892GE17090601
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow matrix fast-flow highly cross-linked 4% beaded agarose

H8280

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow lyophilized powder

C5338

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow

Phenyl-Sepharose CL-4B extent of labeling: ~40 μmol per mL

P7892

Phenyl-Sepharose CL-4B

NHS Act Sepharose™ 4 Fast Flow Cytiva 17-0906-01, pack of 25 mL

GE17090601

NHS Act Sepharose™ 4 Fast Flow

matrix active group

N-hydroxysuccinimide active ester

matrix active group

cyanate or related structures

matrix active group

-

matrix active group

-

form

isopropanol suspension

form

lyophilized powder

form

suspension

form

-

technique(s)

protein array: suitable

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

  • Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation, which gives a high degree of substitution of the selected ligand
  • NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319,H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000389216
    0000335729
    0000389216
    0000335729
    SLCM2411

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    Yoo Min Park et al.
    Talanta, 195, 97-102 (2019-01-11)
    The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
    Woo-Hyeon Byeon et al.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
    Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
    Yoo Min Park et al.
    Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
    We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
    Seulggie Choi et al.
    Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
    Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
    Julia V Hartig et al.
    Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
    In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs
    View All Related Papers

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