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H8163

Sigma-Aldrich

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-HDAC5
MDL number:
MFCD06798461
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~124 kDa

species reactivity

mouse, human, rat

technique(s)

immunocytochemistry: 1-2 μg/mL using cultured NIH-3T3 or HeLa cells
immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human breast carcinoma section
immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant mouse HDAC5
microarray: suitable
western blot (chemiluminescent): 2-4 μg/mL using whole extract of human embryonal kidney 293T cells and whole extract of rat pheochromocytoma PC-12 cells

UniProt accession no.

Q9UQL6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)

General description

Histone Deacetylase 5 (HDAC5) belongs to the class IIa HDAC family. The localization of HDAC5 is both nuclear and cytoplasmic. HDAC5 gene is mapped to human chromosome at 17q21.31.

Specificity

Anti-Histone Deacetylase 5 (HDAC5)(NA-16) recognizes human, rat, and mouse HDAC5.

Immunogen

synthetic peptide corresponding to amino acid residues 384-399 of human HDAC5 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit has been used in:
  • chromatin immunoprecipitation
  • immunostaining
  • immunocytochemistry
  • immunoprecipitation

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Biochem/physiol Actions

Histone Deacetylase 5 (HDAC5) shuttling to the cytoplasm occurs during myogenic differentiation, the nuclear export being stimulated by calcium/ calmodulin-dependent protein kinase (CaMK) phosphorylation at Ser259 and Ser498. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (MEF2) protein, thus repressing expression of MEF2 downstream genes.Overexpression of HDAC5 in various cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. It also plays a key role in many biological processes including the synoviocyte activation, neural regeneration and repair. HDAC5 is highly expressed in breast cancer and contributes to tumor progression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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Functional interaction of histone deacetylase 5 (HDAC5) and lysine-specific demethylase 1 (LSD1) promotes breast cancer progression
Cao C, et al.
Oncogene, 36(1), 133-145 (2017)
HDAC5, a potential therapeutic target and prognostic biomarker, promotes proliferation, invasion and migration in human breast cancer
Li A, et al.
Testing, 7(25), 37966-37966 (2016)
Stress-induced epigenetic transcriptional memory of acetylcholinesterase by HDAC4
Sailaja BS, et al.
Proceedings of the National Academy of Sciences, 109(52), E3687-E3695 (2012)
HDAC5 is a novel injury-regulated tubulin deacetylase controlling axon regeneration
Cho Y and Cavalli V
The Embo Journal, 31(14), 3063-3078 (2012)
Targeting histone deacetylases for cancer therapy: from molecular mechanisms to clinical implications
Li Z and Zhu WG
International Journal of Biological Sciences, 10(7), 757-757 (2014)
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