H8038
Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human, mouse
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: 1-2 μg/mL using cultured 293T cells expressing recombinant human HDAC8
immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant human HDAC8
western blot: 2-4 μg/mL using whole extract of human colon adenocarcinoma SW620 cells and a chemiluminescent detection reagent
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HDAC8(55869)
mouse ... Hdac8(70315)
Related Categories
General description
Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas.
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Immunogen
synthetic peptide corresponding to amino acid residues 352-370 of human HDAC8, conjugated to KLH. The corresponding sequence in mouse differs by one amino acid.
Application
Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit has been used in western blotting, immunohistofluorescence and immunoblotting.
Biochem/physiol Actions
Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. {5} HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Expression of class I histone deacetylases in ipsilateral and contralateral hemispheres after the focal photothrombotic infarction in the mouse brain
Demyanenko S, et al.
Translational Stroke Research, 9(5), 471-483 (2018)
Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function
Doyle K and Fitzpatrick FA
Test, 285(23), 17417-17424 (2010)
Marisela Agudelo et al.
PloS one, 11(6), e0156421-e0156421 (2016-06-02)
Epigenetic mechanisms have been shown to play a role in alcohol use disorders (AUDs) and may prove to be valuable therapeutic targets. However, the involvement of histone deacetylases (HDACs) on alcohol-induced oxidative stress of human primary monocyte-derived dendritic cells (MDDCs)
Profile of class I histone deacetylases (HDAC) by human dendritic cells after alcohol consumption and in vitro alcohol treatment and their implication in oxidative stress: role of HDAC inhibitors trichostatin A and mocetinostat
Agudelo M, et al.
Testing, 11(6), e0156421-e0156421 (2016)
J J Buggy et al.
The Biochemical journal, 350 Pt 1, 199-205 (2000-08-06)
Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDACs are known to have key roles in the regulation of cell proliferation [Brehm
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