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H7891

Sigma-Aldrich

Hexammine cobalt(III) chloride

for use in transformations, X-ray crystallography and NMR

Synonym(s):

Cobalt hexammine trichloride, Hexaamminecobalt trichloride

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About This Item

Linear Formula:
[Co(NH3)6]Cl3
CAS Number:
Molecular Weight:
267.48
EC Number:
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

form

powder

storage temp.

room temp

SMILES string

N.N.N.N.N.N.[Cl-].[Cl-].[Cl-].[Co+3]

InChI

1S/3ClH.Co.6H3N/h3*1H;;6*1H3/q;;;+3;;;;;;/p-3

InChI key

JXBGZYGSWFSYFI-UHFFFAOYSA-K

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General description

Hexammine cobalt(III) is considered an analog of fully solvated magnesium, capable of activating some enzymes that requires magnesium. It is useful in DNA condensation studies.

Application

Suitable for
  • DNA condensation studies
  • induction of transitions of DNA from B to A or Z forms
  • induction of nucleic acid crystal growth
  • stabilization of tertiary tRNA interactions
  • preparation of ligation buffer

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 4 - Carc. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Filip Senigl et al.
Cell reports, 29(12), 3902-3915 (2019-12-19)
Somatic hypermutation (SHM) introduces point mutations into immunoglobulin (Ig) genes but also causes mutations in other parts of the genome. We have used lentiviral SHM reporter vectors to identify regions of the genome that are susceptible ("hot") and resistant ("cold")
J S Kieft et al.
Structure (London, England : 1993), 5(5), 713-721 (1997-05-15)
Solvated metal ions are critical for the proper folding and function of RNA. Despite the importance of these ions, the details of specific metal ion-RNA interactions are poorly understood. The crystal structure of a group I intron ribozyme domain characterized
T Allers et al.
Nucleic acids research, 28(2), e6-e6 (1999-12-22)
The Holliday junction is a central intermediate in genetic recombination. This four-stranded DNA structure is capable of spontaneous branch migration, and is lost during standard DNA extraction protocols. In order to isolate and characterize recombination intermediates that contain Holliday junctions
M C Whitby et al.
The Journal of biological chemistry, 273(31), 19729-19739 (1998-07-25)
The RecG protein of Escherichia coli is a junction-specific DNA helicase that drives branch migration of Holliday intermediates in genetic recombination and DNA repair. The reaction was investigated using synthetic X-junctions. RecG dissociates X-junctions to flayed duplex products, although DNA
K L Kindle
Proceedings of the National Academy of Sciences of the United States of America, 87(3), 1228-1232 (1990-02-01)
By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. The nitrate reductase gene

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