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H7411

Sigma-Aldrich

Anti-HA−FITC antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
MFCD00803873
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

buffered aqueous solution

storage condition

protect from light

concentration

1 mg/mL

technique(s)

immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.

shipped in

wet ice

storage temp.

−20°C

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General description

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:
  • in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
  • in western blotting detection of spindle-associated proteins in Trypanosoma brucei
  • in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

Biochem/physiol Actions

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

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Faithful chromosome segregation in Trypanosoma brucei requires a cohort of divergent spindle-associated proteins with distinct functions
Zhou Q, et al.
Nucleic Acids Research, 46(16), 8216-8231 (2018)
Annalena La Porte et al.
Journal of virology, 90(21), 9889-9904 (2016-08-26)
INI1/hSNF5/SMARCB1/BAF47 is an HIV-specific integrase (IN)-binding protein that influences HIV-1 transcription and particle production. INI1 binds to SAP18 (Sin3a-associated protein, 18 kDa), and both INI1 and SAP18 are incorporated into HIV-1 virions. To determine the significance of INI1 and the
Use of FITC-labeled influenza virus and flow cytometry to assess binding and internalization of virus by monocytes-macrophages and lymphocytes
Nichols JE, et al.
Archives of Virology, 130(3-4), 441-455 (1993)
Yanan Wang et al.
Virology journal, 16(1), 97-97 (2019-08-07)
Transmissible gastroenteritis virus (TGEV), a member of the family Coronaviridae, causes lethal watery diarrhea in piglets. Previous studies have revealed that the coronaviruses develop various strategies to evade the host innate immunity through the inhibition of nuclear factor kappa B
Aortic aneurysm generation in mice with targeted deletion of integrin-linked kinase in vascular smooth muscle cells
Shen D, et al.
Circulation Research, 109(6), 616-628 (2011)
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