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H7140

Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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Select a Size

50 ML
¥2,290.18
5 X 50 ML
¥9,122.20

¥2,290.18

Estimated to ship on2025年4月07日Details

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50 ML
¥2,290.18
5 X 50 ML
¥9,122.20

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

¥2,290.18

Estimated to ship on2025年4月07日Details

Quality Level

100

form

liquid (2x concentrate)

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

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P1415H7782H7033
杂交溶液 for Northern and Southern blotting, liquid (2x concentrate)

H7140

杂交溶液

预杂交溶液 for Northern and Southern blotting, liquid (2x concentrate)

P1415

预杂交溶液

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H7782

杂交溶液

PerfectHyb™ Plus杂交缓冲液 for Northern and Southern blotting, solution

H7033

PerfectHyb™ Plus杂交缓冲液

form

liquid (2x concentrate)

form

liquid (2x concentrate)

form

liquid

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

-

General description

Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

Application

Hybridization Solution has been used to hybridise the nitrocellulose membrane.[1] It has also been used in DNA hybridization.[2]
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Components

Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCM9514
    SLCC2282
    SLBR7356V
    SLBP5108V
    SLBH6970

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    Sindermann JR, et al.
    American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
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    Biosensors And Bioelectronics, 68, 336-342 (2015)
    Sambrook, J., et al.
    Molecular Devices, 9-9 (1989)
    Jürgen R Sindermann et al.
    American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
    To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
    Joel W Slaton et al.
    Molecular cancer research : MCR, 2(12), 712-721 (2005-01-07)
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    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service