H7039
3-Hydroxy-3-methylglutaryl-CoA reductase human
solution
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Assay
≥90% (SDS-PAGE)
Quality Level
form
solution
specific activity
2-8 units/mg protein
mol wt
~76 kDa (for the fusion protein)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HMGCR(3156)
Application
3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:
- in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
- as an antigen to measure anti-HMGCR antibodies by blot analysis
- in HMG-CoA reductase activity assays
- as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot
Biochem/physiol Actions
3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).
Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.
Physical properties
Consists of the HMG CoA reductase catalytic domain fused to GST protein.
Unit Definition
One unit will convert 1.0 μmole of NADPH to NADP+ per minute at 37 °C.
Physical form
Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.
inhibitor
substrate
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Analysis of autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase using different technologies
Musset L, et al.
Journal of immunology research, 2014 (2014)
Myopathy with anti-HMGCR antibodies Perimysium and myofiber pathology
Alshehri A, et al.
Neurology? neuroimmunology & neuroinflammation, 2(4), e124-e124 (2015)
ChemVassa: a new method for identifying small molecule hits in drug discovery
Moldover B, et al.
The Open Medicinal Chemistry Journal, 6, 29-29 (2012)
OP0009 Distinctive Immunofluorescence Pattern in Statin-Associated Autoimmune Myopathy with Anti-HMGCR Autoantibodies
Alvarado-Cardenas M, et al.
Annals of the Rheumatic Diseases, 75, 56-56 (2016)
Marie Hudson et al.
Medicine, 95(44), e5280-e5280 (2016-11-20)
The objective of this study was to investigate the frequency of autoantibodies to hydroxymethylglutaryl coenzyme A reductase (HMGCR) in systemic sclerosis (SSc) and associations with inflammatory myositis and statin exposure.This was a cross-sectional, multicenter study of 306 subjects from the
Articles
Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.
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