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H6390

Sigma-Aldrich

Hemin−Agarose

Type I, saline suspension

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MDL number:
MFCD00163149
UNSPSC Code:
41106500
NACRES:
NA.56

biological source

hemin from bovine

Quality Level

200

type

Type I

form

saline suspension

extent of labeling

≥4 μmol (per ml)

technique(s)

affinity chromatography: suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

epoxy

matrix attachment

carboxyl

matrix spacer

12 atoms

suitability

suitable for chromatography

storage temp.

2-8°C

Application

Hemin Type I-agarose is an agarose conjugate in saline suspension used in affinity chromatography, protein chromatography and specialty resins. Hemin-agarose has been used to evaluate protection against invasion by Gram-negative bacteria.

Physical form

Suspension in 0.5 M NaCl containing preservative

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Where can I find a protocol for using Product H6390, Hemin-Agarose?

    A reference that describes both the preparation and use of hemin-agarose is Analytical Biochemistry, 121, 244-250 (1982).

  4. Can organic solvents be used with Product H6390, Hemin-Agarose?

    Yes, up to 20% ethanol, acetone or neat dimethylformamide may be used.

  5. What is the recommended negative control to use with Product H6390, Hemin-Agarose?

    Product 4B200 (Sepharose 4B) is a good negative control to use for H6390.

  6. What is the recommended pH range for Product H6390, Hemin-Agarose?

    The pH range that the hemin agarose can be used at (and at which the hemin is expected to be stable) is 6.5-9.0.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Inducible glutathione S-transferase (IrGST1) from the tick Ixodes ricinus is a haem-binding protein
Perner J, et al.
Insect Biochemistry and Molecular Biology, 95, 44-54 (2018)
C S Bracken et al.
Journal of bacteriology, 181(19), 6063-6072 (1999-09-28)
The abilities of two bacterial active heme transporters, HmbR of Neisseria meningitidis and HemR of Yersinia enterocolitica, to use different heme sources were compared. While HmbR-expressing cells used only hemoglobin (Hb) and heme, HemR-expressing bacteria were able to grow on
Hye-Jung Lee et al.
Journal of experimental botany, 63(16), 5967-5978 (2012-09-20)
The Arabidopsis thaliana L. SOUL/haem-binding proteins, AtHBPs belong to a family of five members. The Arabidopsis cytosolic AtHBP1 (At1g17100) and AtHBP2 (At2g37970) have been shown to bind porphyrins and metalloporphyrins including haem. In contrast to the cytosolic localization of these
Swapna Asuthkar et al.
Infection and immunity, 75(9), 4582-4591 (2007-06-20)
In an earlier study, based on the ferric enterobactin receptor FepA of Escherichia coli, we identified and modeled a TonB-dependent outer membrane receptor protein (LB191) from the genome of Leptospira interrogans serovar Lai. Based on in silico analysis, we hypothesized
S Yamamoto et al.
FEMS microbiology letters, 128(2), 195-200 (1995-05-01)
Several clinical isolates of Vibrio parahaemolyticus were examined for their ability to utilize either hemin or hemoglobin as a sole source of iron. Both compounds appeared to be equally good iron sources. Maximum growth was obtained at 5 microM hemin
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