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H6383

Sigma-Aldrich

Hydroxyalkoxypropyl-Dextran

Type IX

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About This Item

MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

type

Type IX

Quality Level

form

beads

technique(s)

liquid chromatography (LC): suitable

storage temp.

2-8°C

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Application

Hydroxyalkoxypropyl-Dextran is used for liquid chromatography, protein chromatography, and gel filtration chromatography. Hydroxyalkoxypropyl-Dextran has been used to develop a nondestructive method for effective removal of lipids from small quantities of aqueous protein as well as to develop a highly specific method for the determination of cortisol in human serum and urine.
Lipophilic, hydrophobic gel for liquid chromatography.

Other Notes

Hydroxypropyl beaded dextran (Lipophilic Sephadex, LH-20-100), substituted with long chain alkyl ethers.
Substituted approx. 50% by weight with alkyl chains of length C15-C18

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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D Apter et al.
Clinica chimica acta; international journal of clinical chemistry, 63(2), 139-148 (1975-09-01)
A highly specific method for the determination of cortisol in human serum and urine is described. The sample is first extracted with diethyl ether/ethyl acetate (1 : 1, by vol.), then chromatographed on a highly lipophilic derivative of Sephadex (hydroxyalkoxypropyl
J B Lowe et al.
The Journal of biological chemistry, 262(12), 5931-5937 (1987-04-25)
Rat intestinal fatty acid-binding protein (I-FABP) is an abundant, 15,124-Da polypeptide found in the cytosol of small intestinal epithelial cells (enterocytes). It is homologous to rat liver fatty acid-binding protein (L-FABP), a 14,273-Da cytosolic protein which is found in enterocytes
U Kragh-Hansen
Analytical biochemistry, 210(2), 318-327 (1993-05-01)
A simple and nondestructive method was developed for effective removal of lipids, such as long-chain fatty acids and steroids, from small quantities (2-10 mg) of aqueous protein. The procedure operates with a high recovery of protein (97%) and was elaborated

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