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H5912

Sigma-Aldrich

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

H2Ax Antibody, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, Anti-H2AXS139p

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Select a Size

10 MG
CN¥1,177.86
50 MG
CN¥4,132.48
100 MG
CN¥7,415.09

CN¥1,177.86

Available to ship onApril 27, 2025Details

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10 MG
CN¥1,177.86
50 MG
CN¥4,132.48
100 MG
CN¥7,415.09

About This Item

MDL number:
MFCD04974783
UNSPSC Code:
12352203
NACRES:
NA.43

CN¥1,177.86

Available to ship onApril 27, 2025Details

Request a Bulk Order

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 15 kDa

species reactivity

mouse, human

technique(s)

microarray: suitable
western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine

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Eppendorf® epT.I.P.S. box volume range 50-1000 μL

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Eppendorf® epT.I.P.S. box volume range 2-200 μL

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Eppendorf® epT.I.P.S. box volume range 100-5000 μL

Z640271

Eppendorf® epT.I.P.S. box

feature

barrier: no

feature

barrier: no

feature

barrier: no

feature

barrier: no

manufacturer/tradename

Eppendorf® 0030 073.100, Eppendorf® 022491351

manufacturer/tradename

Eppendorf® 0030 073.002, Eppendorf® 022491300

manufacturer/tradename

Eppendorf® 0030 073.061, Eppendorf® 022491334

manufacturer/tradename

Eppendorf® 0030 073.169, Eppendorf® 022491385

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

blue rack

material

dark gray rack

material

yellow rack

material

violet rack

packaging

pack of 96 (includes 1 reusable box)

packaging

pack of 96 (includes 1 reusable box)

packaging

pack of 96 (includes 1 reusable box)

packaging

pack of 24 (includes 1 reusable box)

L

71 mm

L

34 mm

L

53 mm

L

120 mm

General description

H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.

Immunogen

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.
synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer139).

Application

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

Biochem/physiol Actions

H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000409212
    0000386117
    0000336889
    0000409212
    0000386117

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Kalyan Mahapatra et al.
    Scientific reports, 11(1), 11659-11659 (2021-06-04)
    As like in mammalian system, the DNA damage responsive cell cycle checkpoint functions play crucial role for maintenance of genome stability in plants through repairing of damages in DNA and induction of programmed cell death or endoreduplication by extensive regulation
    Resveratrol decreases Rad51 expression and sensitizes cisplatin-resistant MCF-7 breast cancer cells
    Leon-Galicia I, et al.
    Oncology Reports, 39(6), 3025-3033 (2018)
    H2AX: functional roles and potential applications
    Dickey J, et al.
    Chromosoma, 118(6), 683-692 (2009)
    Genomic instability in mice lacking histone H2AX
    Celeste A, et al.
    Science (New York, N.Y.), 296(5569), 922-927 (2002)
    ATM-dependent DNA damage-independent mitotic phosphorylation of H2AX in normally growing mammalian cells
    McManus KJ and Hendzel MJ
    Molecular Biology of the Cell, 16(10), 5013-5025 (2005)
    View All Related Papers

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