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General description
Elution buffer is a major solvent in affinity chromatography. Elution buffer is used to wash away unbound proteins at first and at a greater concentration it releases the desired protein from the ligand. It is important that the elution buffer works quickly without changing the function or activity of the desired protein.
Application
HIS-Select® Elution Buffer has been used in the elution of recombinant coagulase protein and tomoregulin
Physical form
Contains 250 mM Imidazole, 0.3 M sodium chloride, 50 mM sodium phosphate, pH 8.0.
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Hyun-Seok Hong et al.
Journal of Alzheimer's disease : JAD, 48(3), 731-743 (2015-09-25)
Amyloid-β (Aβ) protein causes neurotoxicity and its abnormal aggregation into amyloid is a pathological hallmark of Alzheimer's disease (AD). Cellular proteins able to interact with Aβ or its precursor, AβPP (amyloid-β protein precursor), may regulate Aβ production and neurotoxicity. We
Affinity Chromatography
Scouten, William H
Chromatography (1981)
Alaa H Sewid et al.
Infection and immunity, 86(8) (2018-06-13)
Coagulase activation of prothrombin by staphylococcus induces the formation of fibrin deposition that facilitates the establishment of infection by Staphylococcus species. Coagulase activity is a key characteristic of Staphylococcus pseudintermedius; however, no coagulase gene or associated protein has been studied
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PloS one, 9(11), e113294-e113294 (2014-11-20)
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