H5388
Hyaluronic acid sodium salt from rooster comb
avian glycosaminoglycan polysaccharide
Synonym(s):
Poly(β-glucuronic acid-[1→3]-β-N-acetylglucosamine-[1→4]), alternating
About This Item
biological source
chicken (rooster comb)
Quality Level
form
powder
impurities
≤2% protein (Lowry)
color
white
solubility
water: ~5 g/L
cation traces
Na: 3-7%
storage temp.
−20°C
InChI
1S/C28H44N2O23.Na/c1-5(33)29-9-18(11(35)7(3-31)47-25(9)46)49-28-17(41)15(39)20(22(53-28)24(44)45)51-26-10(30-6(2)34)19(12(36)8(4-32)48-26)50-27-16(40)13(37)14(38)21(52-27)23(42)43;/h7-22,25-28,31-32,35-41,46H,3-4H2,1-2H3,(H,29,33)(H,30,34)(H,42,43)(H,44,45);/q;+1/t7-,8-,9-,10-,11-,12-,13+,14+,15-,16-,17-,18-,19-,20+,21+,22+,25-,26+,27-,28-;/m1./s1
InChI key
YWIVKILSMZOHHF-QJZPQSOGSA-N
Related Categories
Application
- as a component of bi-layer gelatin scaffold
- as a vehicle for release of gentamicin during left ear surgery in guinea pigs
- in hyaluronic acid binding assay in human embryonic kidney cell line 293T
Biochem/physiol Actions
Other Notes
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the molecular weight of Hyaluronic acid, Product H5388?
Product No. H5388 is a linear heteropolysaccharide with repeating 3-O-(β-D-glucuronido)-N-acetyl-D-glucosamine units linked by β-(1-->4)) hexosaminidic bonds. The molecular weight of this subunit has not been determined, but could be calculated from the information above. Molecular weights of hyaluronic acids vary from 0.1-10 million, depending on the source. The hyaluronic acid from rooster comb is expected to have a molecular weight of 1-4 million.
What is the purity of Hyaluronic acid, Product H5388?
Sigma does not perform purity testing on this product due to the variable molecular weight and degradation that may occur in native glycosaminoglycans. This product is tested for activity and protein content which provide information regarding impurities and suitability.
What is the solubility of Hyaluronic acid, Product H5388?
For Sigma's analytical test, 15 mg of this product is dissolved in 5 mL of 0.3 M phosphate buffer, pH 5.35 at 25°C (i.e., 3 mg/mL).
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Articles
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protocols
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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