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H5042

Sigma-Aldrich

Cytochrome P450 3A4 human

recombinant, expressed in E. coli

Synonym(s):

Cytochrome P450, purified, soluble, 1,4-cineole 2-exo-monooxygenase, Albendazole sulfoxidase, CYP3A3, CYP3A4, Cytochrome P450 NF-25, Cytochrome P450-PCN1

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About This Item

CAS Number:
9035-51-2
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

human

Quality Level

200

recombinant

expressed in E. coli

description

purified, soluble

Assay

3.5—25 mg protein/mL (Bradford)

form

liquid

specific activity

12-200 units/mg protein

mol wt

57318.7 g/mol

concentration

3.5-25 mg protein/mL (protein)
50-150 nmol/mL (P450)
6-14 nmol/mg protein (specific content)

technique(s)

cell based assay: suitable

solubility

water: soluble

NCBI accession no.

NP_059488.2

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP3A4(1576)

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General description

Research area: IMMUNO AND CKS

Cytochrome P450 3A4 (CYP3A4) indicates a mammalian enzyme (CYP) family 3, subfamily A, gene 4. There are 57 CYP genes and 33 pseudogenes in humans, organized into 18 families and 42 subfamilies. Among these, the 3A4 isoform (CYP3A4) is the most prevalent and is primarily found in the liver and gastrointestinal tract.

Application

Cytochrome P450 3A4 human has been used in circular dichroism analysis.

Biochem/physiol Actions

Cytochrome P450 3A4 (CYP3A4) is one of the isoforms of cytochrome P450 (P450s). Cytochrome P450s (P450s) plays a vital role in processing endogenous compounds and xenobiotics, including pollutants, environmental compounds, and drug molecules. CYP3A4 oxidizes more than 90% of marketed drugs through various metabolic processes.CYP3A4 mRNA expression is upregulated in pediatric Crohn’s disease. Moreover, it provides high tolerance against herbicides such as mefenacet, amiprofos-methyl, pyributicarb, norflurazon, trifluralin, pendimethalin, chlorotoluron, and chloroacetamides.

Unit Definition

One unit will convert 1.0 nanomole of testosterone to 6-beta-testosterone per minute at pH 7.4 at 37 °C.

Other Notes

Supplied as purified soluble proteins, rather than microsomal preparations.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Understanding the mechanism of cytochrome P450 3A4: recent advances and remaining problems
Sevrioukova IF, et al.
Journal of Separation Science, 42(9), 3116-3126 (2013)
Understanding the mechanism of cytochrome P450 3A4: recent advances and remaining problems
Sevrioukova IF, et al.
Journal of Separation Science, 42(9), 3116-3126 (2013)
Modulation of Carbon Nanotubes' Perturbation to the Metabolic Activity of CYP3A4 in the Liver
Zhang Y, et al.
Advances in Functional Materials, 26(6), 841-850 (2016)
Elisavet Stavropoulou et al.
Frontiers in immunology, 9, 89-89 (2018-02-16)
Cytochromes are expressed in many different tissues of the human body. They are found mostly in intestinal and hepatic tissues. Cytochromes P450 (CYPs) are enzymes that oxidize substances using iron and are able to metabolize a large variety of xenobiotic
Pharmacokinetic-pharmacodynamic consequences and clinical relevance of cytochrome P450 3A4 inhibition
Dresser, et al.
Clinical Pharmacokinetics, 38(1), 41-57 (2000)
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