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Merck
CN

H4385

Hanks′ Balanced Salt solution

10 ×, Modified, without calcium, magnesium or sodium bicarbonate

Synonym(s):

HBSS

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About This Item

NACRES:
NA.47
UNSPSC Code:
12352107
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Quality Level

concentration

10 ×

pH

5.8-6.0

application(s)

hematology
histology

storage temp.

room temp

General description

Hanks′ Balanced Salt solution (HBSS) is a cell and tissue culture solution. It is widely recommended for cell maintenance and conservation of tissues for a longer period. In addition, HBSS is used as a wet storage media for avulsed teeth, as it has the ideal osmolality and physiological pH for preserving the vitality of cells.

Application

Hanks′ Balanced Salt solution (HBSS) has been used for the elimination of the epithelial layer from the leukocytes and lymphocytes from the colon cells. It has also been used for the isolation of quail spermatozoa

Packaging

Contains: NaCl, 80 g/L; KCl, 4 g/L; glucose, 10 g/L; KH2PO4 600 mg/L; Na2HPO4 475 mg/L and phenol red, 170 mg/L

Other Notes

pH 6.9 when diluted according to instructions. Osmolality 240-250 mOsm/Kg.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Shusei Mizushima et al.
Methods in molecular biology (Clifton, N.J.), 1650, 243-257 (2017-08-16)
A characteristic biological property of avian gamete (e.g., extremely large egg and polyspermic fertilization) does not allow the direct observation of sperm-egg interactions in vitro, but recent research advances make it possible to manipulate the gamete in vitro. Here, we
Wilson Roberto Poi et al.
Brazilian dental journal, 24(5), 437-445 (2014-01-30)
Dental avulsion is the most severe type of traumatic tooth injuries because it causes damage to several structures and results in the complete displacement of the tooth from its socket in the alveolar bone. The ideal situation is to replant
Athina-Myrto Chioni et al.
Methods in molecular biology (Clifton, N.J.), 1487, 255-267 (2016-12-08)
Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic



Global Trade Item Number

SKUGTIN
H4385-100ML04061833795163
H4385-6X100ML04061833795170