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H3537

Sigma-Aldrich

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Synonym(s):

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

CAS Number:
7365-45-9
MDL number:
MFCD00006158
UNSPSC Code:
12161700
PubChem Substance ID:
329815199
NACRES:
NA.25

product name

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grade

BioPerformance Certified

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
 DNase, RNase, Protease, Nickase, free
 endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
  • To recover purified ribonucleotide
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
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Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
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