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H3034

Sigma-Aldrich

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-HD3

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About This Item

MDL number:
MFCD02096545
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
immunoprecipitation (IP): 1:2,500 using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:10,000 using nuclear extract from HeLa human epithelioid carcinoma cells
western blot: 1:2,000 using whole extract of PC-12 rat pheochromocytoma cell line

UniProt accession no.

O15379

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HDAC3(8841)
mouse ... Hdac3(15183)
rat ... Hdac3(84578)

General description

Histone deacetylase 3 (HDAC3) is an epigenetic modifier and is expressed in various tissues. It belongs to the class I subfamily of histone deacetylases. The HDAC3 gene is mapped on to human chromosome 5q31.3.

Specificity

Anti-Histone Deacetylase 3 specifically recognises histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin.

Immunogen

synthetic peptide corresponding to amino acids 411-428 of human and chicken HDAC3.

Application

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blotting
  • immunoprecipitation

Biochem/physiol Actions

Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
Histone deacetylase 3 (HDAC3) acts as a transcriptional modulator of inflammatory genes. It deacetylates lysine residues of histones. HDAC3 associates with nuclear receptor co-repressor (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRTs) and activates the complex. This protein participates in interleukin-1 expression and inflammatory responses.

Physical form

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Xiaohong Xiang et al.
Clinical science (London, England : 1979), 136(1), 45-60 (2021-12-18)
Cold storage/rewarming is an inevitable process for kidney transplantation from deceased donors, which correlates closely with renal ischemia-reperfusion injury (IRI) and the occurrence of delayed graft function. Histone deacetylases (HDAC) are important epigenetic regulators, but their involvement in cold storage/rewarming
Farah H Bardai et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(29), 11833-11838 (2013-07-19)
Huntington's disease (HD) is caused by a polyglutamine expansion within the huntingtin (Htt) protein. Both loss of function of normal Htt and gain of a toxic function by the polyglutamine-expanded mutant Htt protein have been proposed to be responsible for
Histone deacetylase 3 regulates the inflammatory gene expression programme of rheumatoid arthritis fibroblast-like synoviocytes.
Angiolilli C, et al.
Annals of the Rheumatic Diseases, 76(1), 277-285 (2017)
HDAC3 role in medication consumption in medication overuse headache patients: a pilot study.
Pisanu C, et al.
Human Genomics, 9, 30-30 (2015)
Histone Deacetylase-1 (HDAC1) Is a Molecular Switch between Neuronal Survival and Death
Bardai FH, et al.
The Journal of biological chemistry, 287(42), 35444-35453 (2012)
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