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H1506

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Synonym(s):

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

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About This Item

CAS Number:
9028-56-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
MFCD00130298
EC Number:
1.1.1.50 (BRENDA, IUBMB)

Key Documents

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Product Name

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni, ≥15 units/mg protein

biological source

bacterial (Pseudomonas testosteroni)

form

lyophilized solid

specific activity

≥15 units/mg protein

purified by

chromatography

composition

Protein, ≥25%

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

Quality Level

200

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Application

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Biochem/physiol Actions

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Other Notes

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000431186
0000431186
0000421205
0000421205
0000202600

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Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Xiang Li et al.
Archives of pharmacal research, 30(7), 812-815 (2007-08-21)
Two novel lactones, Cephalosporolides H (1) and 1 (2), were isolated from the lyophilized culture broth of the marine-derived fungus, Penicillium sp. The structures were elucidated on the basis of extensive 1D- and 2D-NMR, as well as HRESI-MS spectroscopic analyses.
Brandon Bartling et al.
The Analyst, 134(5), 973-979 (2009-04-22)
An electrochemical sensor, based on thick-film screen-printed Ir/C working and counter electrodes, was developed for the detection of total bile acid concentration in a physiological fluid for potential patient management in patients with liver disease. Current electrochemical methods of detecting
Frank Hoffmann et al.
Journal of biotechnology, 129(1), 131-139 (2007-01-30)
3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni belongs to the short chain dehydrogenase/reductase (SDR) protein superfamily and catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid. The enzyme also mediates the carbonyl reduction of
Chiaki Hidai et al.
Bioprocess and biosystems engineering, 32(5), 569-573 (2008-11-20)
Developing methods that result in targeting of therapeutic molecules in gene therapies to target tissues has importance, as targeting can increase efficacy and decrease off target-side-effects. Work from my laboratory previously showed that the extracellular matrix protein Del1 is organized
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