H1506
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni
≥15 units/mg protein
Synonym(s):
3α-Hydroxysteroid:NAD(P)+ oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
Specific activity:
≥15 units/mg protein
Biological source:
bacterial (Pseudomonas testosteroni)
biological source
bacterial (Pseudomonas testosteroni)
form
lyophilized solid
specific activity
≥15 units/mg protein
purified by
chromatography
composition
Protein, ≥25%
foreign activity
β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%
storage temp.
−20°C
Quality Level
Application
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
- in the preparation of composite electrode platform to for the determination of androsterone
- in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
- for total bile salts assay using an enzymatic method
Biochem/physiol Actions
3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.
Physical form
Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol
Other Notes
One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Enzyme biosensor for androsterone based on 3alpha-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode
Mundaca RA, et al.
Talanta, 99(, 697-702 (2012)
Phytosterol ester processing in the small intestine; impact on cholesterol availability for absorption and chylomicron cholesterol incorporation in healthy humans
Amiot MJ, et al.
Journal of Lipid Research, jlr-M013730 (2011)
Bioluminescent assay for total bile acids in serum with use of bacterial luciferase.
Styrelius I, et al.
Clinical Chemistry, 29(6), 1123-1127 (1983)
Anne Z Steiner et al.
The Journal of clinical endocrinology and metabolism, 93(4), 1298-1303 (2008-02-07)
Dihydrotestosterone (DHT), the primary active androgen in peripheral target tissues, is metabolized by 3alpha-hydroxysteroid dehydrogenase type III (3alpha-HSD), encoded by the AKR1C2 gene, forming 5alpha-androstane-3alpha,17beta-diol (3alpha-diol). 3alpha-HSD may play a role in the pathogenesis of hirsutism. Our objective was to
Laurence Meyer et al.
Neurobiology of disease, 30(1), 30-41 (2008-02-23)
Identification of cellular targets pertinent for the development of effective therapies against pathological pain constitutes a difficult challenge. We combined several approaches to show that 3alpha-hydroxysteroid oxido-reductase (3alpha-HSOR), abundantly expressed in the spinal cord (SC), is a key target, the
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