Skip to Content
Merck
CN
All Photos(2)

Documents

Safety Information

H0267

Sigma-Aldrich

Hemoglobin A0, Ferrous Stabilized human

lyophilized powder

Synonym(s):

Hemoglobin A0 Ferrous Stabilized, Hemoglobin-A0

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

CAS Number:
54651-57-9
EC Number:
259-278-9
MDL number:
MFCD00131287
UNSPSC Code:
12352202
eCl@ss:
42030116
NACRES:
NA.61

biological source

human

Quality Level

200

Assay

97-100% (agarose gel electrophoresis)

form

lyophilized powder

technique(s)

immunofluorescence: suitable

solubility

H2O: soluble 20 mg/mL

suitability

suitable for electrophoresis and chromatography standard

UniProt accession no.

P68871

storage temp.

−20°C

Gene Information

human ... HBB(3043)

Looking for similar products? Visit Product Comparison Guide

General description

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.
has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).

Application

Hemoglobin A0, ferrous stabilized human has been used:
  • as the non-fluorescent absorber in the preparation of tissue-mimicking phantoms
  • to see how coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype
  • to study the interference of hemoglobin (Hb) A0, HbA2, and HbS in the assay of HbF

Hemoglobin A0 was used to see how coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype. It was used in the determination of fetal hemoglobin by time-resolved immunofluorometric assay.

Biochem/physiol Actions

Variation in the haemoglobin (Hb) encoding genes results in haemoglobinopathies such as thalassaemia and atypical structural variants like Hb Lepore and HbE.

Packaging

Package size indicates the amount of hemoglobin as determined by the procedure of Drabkin, D.L., J. Biol. Chem., 164, 703 (1946).

Reconstitution

When reconstituted with buffer, gives >85% ferrous hemoglobin.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the structure of Product H0267, Hemoglobin A0?

    Hemoglobin A0 is the major hemoglobin protein of adult hemoglobin. It is a tetrameric protein consisting of 2 alpha and 2 beta chains, each of which contains a heme moiety and iron.

  4. What is the molecular weight of H0267 Hemoglobin A0?

    Sigma has not evaluated the molecular weight of hemoglobin.  Based on the reported sequence and including the weight of the 4 heme groups and 4 iron atoms, the molecular weight is approximately 64,677 Daltons. The Structure and Action of Proteins, p. 52, Harper and Row (1969).

  5. How can Product H0267, Hemoglobin A0, be dissolved?

    Hemoglobin is soluble in water at 20 mg/mL.  Solutions in phosphate buffer at about neutral pH can also be prepared.

  6. What is the solution stability of Product H0267, Hemoglobin A0?

    Solutions of ferrous hemoglobin are not stable indefinitely due to the likely autoxidation of the heme group leading to the formation of the ferric derivative (methemoglobin). It is recommended to prepare solutions fresh in deaerated water or buffer.  If necessary, store such prepared solutions no more than 1-2 days at 2-8°C.

  7. Is there methemoglobin in Product H0267, Hemoglobin A0?

    The product may contain a small amount of methemoglobin as described on the specification sheet.

  8. Can Product H0267, Hemoglobin A0, be used in functional studies involving the binding and release of oxygen as a ligand?

    Sigma has not validated this hemoglobin for use in functional studies. The iron in the heme moiety is in the +2 state.  However, this does not mean that this hemoglobin will behave necessarily like native hemoglobin nor be useful for functional studies of the molecule.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Motasam Majedy et al.
Journal of biomedical optics, 27(7) (2021-12-02)
For optical methods to accurately assess hemoglobin oxygen saturation in vivo, an independently verifiable tissue-like standard is required for validation. For this purpose, we propose three hemoglobin preparations and evaluate methods to characterize them. To spectrally characterize three different hemoglobin
Randi E Gislefoss et al.
Biopreservation and biobanking, 19(6), 525-530 (2021-10-07)
Background: During sampling and processing, blood samples can be affected by hemolysis. Information is lacking regarding hemolysis for biobank samples. There is a need for a method that can easily measure hemoglobin as an indicator of hemolysis in stored samples
Seung Yup Lee et al.
Science advances, 6(47) (2020-11-22)
Pancreatic cancer is one of the deadliest cancers, with a 5-year survival rate of <10%. The current approach to confirming a tissue diagnosis, endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), requires a time-consuming, qualitative cytology analysis and may be limited because of
Vivide Tuan-Chyan Chang et al.
Neoplasia (New York, N.Y.), 11(4), 325-332 (2009-03-25)
Cervical cancer is the second most common female cancer worldwide. The ability to quantify physiological and morphological changes in the cervix is not only useful in the diagnosis of cervical precancers but also important in aiding the design of cost-effective
Screening and diagnosis for haemoglobin disorders
Prevention of Thalassaemias and Other Haemoglobin Disorders, 1 (2013)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service