GW20071F
Anti-β-Galactosidase antibody produced in chicken
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Beta-Galactosidase
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About This Item
biological source
chicken
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
bacteria
manufacturer/tradename
Genway 15-288-20071F
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Immunogen
Immunogen Sequence: GI # 26246355, sequence 1-1024
Recombinant β-galactosidase [Escherichia coli CFT073]
Application
Anti-β-Galactosidase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.
Biochem/physiol Actions
The lac operon (lactose operon) is an essential operon used for the transport and metabolism of lactose in Escherichia coli and some enteric bacteria. It has three adjacent structural genes, lacZ, lacY and lacA. The lacZ gene encodes β-galactosidase. Escherichia coli β-galactosidase is mostly used as a reporter enzyme. It is produced under oxidizing conditions in the cytoplasm of an Origami strain. β-galactosidase occurs in a disulfide bond. At the active site of β-galactosidase a loop opens and closes during catalysis.
Physical form
Solution in phosphate buffered saline containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Rebecca E Harvey et al.
Endocrinology, 158(7), 2179-2189 (2017-04-04)
Ghrelin plays a key role in appetite, energy homeostasis, and glucose regulation. Recent evidence suggests ghrelin suppresses inflammation in obesity; however, whether this is modulated by the acylated and/or des-acylated peptide is unclear. We used mice deficient in acylated ghrelin
Joaquin Seras-Franzoso et al.
Applied and environmental microbiology, 78(7), 2376-2385 (2012-01-31)
Escherichia coli β-galactosidase is probably the most widely used reporter enzyme in molecular biology, cell biology, and biotechnology because of the easy detection of its activity. Its large size and tetrameric structure make this bacterial protein an interesting model for
Larisa J Jancewicz et al.
Archives of biochemistry and biophysics, 517(2), 111-122 (2011-12-14)
A loop (residues 794-803) at the active site of β-galactosidase (Escherichia coli) opens and closes during catalysis. The α and β carbons of Ser-796 form a hydrophobic connection to Phe-601 when the loop is closed while a connection via two
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