GERPN2610
MMP-1, Human, Biotrak™ ELISA
Cytiva RPN2610, pack of 1 ea, size 96 wells
Sign Into View Organizational & Contract Pricing
All Photos(1)
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
shelf life
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN2610
size
96 wells
shipped in
dry ice
storage temp.
−20°C
General description
MMP-1, Human, Biotrak™ Assay.
Application
The MMP-1 Human Biotrak™ ELISA system has been specifically designed for research purposes. MMP-1 may be measured in the range 6.25-100 ng/mL.
The assay is based on a two site ELISA ‘sandwich′ format. Standards and samples are incubated in microplate wells pre-coated with anti-MMP-1 antibody. Any MMP-1 present will be.
bound to the wells, other components of the sample being removed by washing and aspiration. A second polyclonal antibody to MMP-1 is then bound to the MMP-1. Any excess is removed by washing and aspiration. The second antibody bound to the wells is detected using donkey anti-rabbit horseradish peroxidase. The amount of peroxidase bound to each well is determined by the addition of TMB ‘ready-to-use′ substrate. The reaction is stopped by addition of an acid solution, and the resultant color read at 450 nm in a microplate spectrophotometer. The concentration of MMP-1 in a sample is determined by interpolation from a standard curve.
Each pack contains reagent for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate.
The assay is based on a two site ELISA ‘sandwich′ format. Standards and samples are incubated in microplate wells pre-coated with anti-MMP-1 antibody. Any MMP-1 present will be.
bound to the wells, other components of the sample being removed by washing and aspiration. A second polyclonal antibody to MMP-1 is then bound to the MMP-1. Any excess is removed by washing and aspiration. The second antibody bound to the wells is detected using donkey anti-rabbit horseradish peroxidase. The amount of peroxidase bound to each well is determined by the addition of TMB ‘ready-to-use′ substrate. The reaction is stopped by addition of an acid solution, and the resultant color read at 450 nm in a microplate spectrophotometer. The concentration of MMP-1 in a sample is determined by interpolation from a standard curve.
Each pack contains reagent for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate.
Features and Benefits
- Measures total MMP-1.
- Specific for human MMP-1.
- Precise and accurate measurement.
- Non-isotopic protocol.
- Ready to use substrate.
- Same day protocol.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak is a trademark of Cytiva
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service