GERPN203B
Hybond®-N+ hybridization membranes
W × L 20 cm × 3 m, roll, pack of 1 ea
Synonym(s):
Hybond-N+ Membranes, Hybridization Membranes
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About This Item
UNSPSC Code:
41105339
NACRES:
NB.22
Recommended Products
material
positively charged nylon
roll
form
solid
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN203B
W × L
20 cm × 3 m
color
White
pore size
0.45 μm pore size
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General description
Hybond™N+ is a high sensitivity positively charged nylon membrane for DNA and RNA blotting with nucleic acid binding capacity and high tensile strength.
Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.
Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.
Application
Hybond™-N+ hybridization membranes are ideal to use with:
- Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
- All membrane arrays with a high density.
- Applications that need high sensitivity and/or probing.
Features and Benefits
- On both sides, all Hybond™membranes are identicalIncompatible with alkali blotting and fixation.
- To ensure that only the most consistent product reaches the user, production runs are carefully monitored, and the product is thoroughly screened.
- Specially designed for life science applications.
- In conventional southern blotting, produces excellent sensitivity.
- To prevent high background sides, nylon needs to be extensively blocked before being detected with antibodies due to its high protein binding capacity.
- Not suitable for western blotting.
Legal Information
Hybond is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
Regulatory Information
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