GERPN1210B
Hybond®-N+ hybridization membranes
W × L 12 cm × 10 cm, sheets, pack of 20 ea
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About This Item
UNSPSC Code:
41105339
NACRES:
NB.22
material
positively charged nylon
sheets
form
solid
packaging
pack of 20 ea
manufacturer/tradename
Cytiva RPN1210B
W × L
12 cm × 10 cm
color
White
pore size
0.45 μm pore size
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General description
Hybond™N+ is a high sensitivity positively charged nylon membrane for DNA and RNA blotting with nucleic acid binding capacity and high tensile strength.
Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.
Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.
Application
Hybond™-N+ hybridization membranes are ideal to use with:
- Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
- All membrane arrays with a high density.
- Applications that need high sensitivity and/or probing.
Features and Benefits
- On both sides, all Hybond™membranes are identicalIncompatible with alkali blotting and fixation.
- To ensure that only the most consistent product reaches the user, production runs are carefully monitored, and the product is thoroughly screened.
- Specially designed for life science applications.
- In conventional southern blotting, produces excellent sensitivity.
- To prevent high background sides, nylon needs to be extensively blocked before being detected with antibodies due to its high protein binding capacity.
- Not suitable for western blotting.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
Hybond is a registered trademark of Cytiva
Regulatory Information
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Certificates of Analysis (COA)
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Huiwen Song et al.
Autophagy, 15(8), 1419-1437 (2019-03-15)
N6-methyladenosine (m6A) mRNA modifications play critical roles in various biological processes. However, no study addresses the role of m6A in macroautophagy/autophagy. Here, we show that m6A modifications are increased in H/R-treated cardiomyocytes and ischemia/reperfusion (I/R)-treated mice heart. We found that
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