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Safety Information

GE29-0486-53

Sigma-Aldrich

StrepTrap™ High Performance prepacked chromatography column

pkg of 1 × 1 mL, prepacked chromatography column

Synonym(s):

StrepTrap™ High Performance

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

StrepTactin

grade

for chromatography

packaging

pkg of 1 × 1 mL

manufacturer/tradename

Cytiva 29-0486-53

availability

not available in North America

storage condition

(20% Ehtanol)

parameter

42 psi (H2O at room temperature.)

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

working range

7

capacity

~6 mg binding capacity (Strep II-tagged protein/ml medium)

storage temp.

2-8°C

General description

StrepTrap HP is a ready to use HiTrap column prepacked with StrepTactin Sepharose High Performance for purifying Strep-tag II recombinant proteins
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Features and Benefits

  • Highly pure Strep-tag II recombinant proteins eluted in concentrated form and small volumes.
  • Physiological conditions and mild elution preserves the activity of the target protein.
  • Convenient, time-saving operation and reproducible results.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

StrepTrap is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Articles

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

Purification using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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