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Key Documents

Safety Information

GE29-0005-93

His Spintrap™ TALON®

Cytiva 29-0005-93

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100 μL
CN¥5,074.24

CN¥5,074.24

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100 μL
CN¥5,074.24

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

CN¥5,074.24

Please contact Customer Service for Availability

ligand

TALON® Superflow Cobalt-IMAC

manufacturer/tradename

Cytiva 29-0005-93

packaging

pkg of 50 x 100 μL

bed volume

100 μL

matrix

6% cross-linked agarose

particle size

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

capacity

≤1 mg binding capacity(histidine-tagged)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

storage temp.

2-8°C

Related Categories

General description

His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Application

His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Features and Benefits

  • Suitable for purification of multiple samples in parallel, such as screening.
  • Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • With a microcentrifuge, one run takes about 10 minutes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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    Articles

    Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

    This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

    Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

    This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

    Protocols

    Purification of Histidine-Tagged Proteins using TALON® Superflow

    This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service