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LPLC: suitable
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Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocols
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
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