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GE28-9782-43

HiScreen™ Blue Fast Flow

Cytiva 28-9782-43

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

Cibacron Blue

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9782-43

storage condition

(20% Ethanol, 0.1 M potassium phosphate)
 (20% Ethanol, 0.9% NaCl, 1 mM Ca2₊ and 1 mM Mn2₊)

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

column I.D.

7.7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

3-13

working range

4-12

suitability

suitable for bioprocess medium

storage temp.

2-8°C

Related Categories

General description

HiScreen™ columns prepacked with Blue Sepharose™ Fast FLow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.

Application

HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).

Features and Benefits

  • Can be used for depletion of albumin.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

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Articles

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocols

Purification or Removal of Albumin

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service