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GE28-9670-70

Protein G Mag Sepharose Xtra

Cytiva 28-9670-70, pack of 5 × 1 mL

Synonym(s):

Protein G magnetic beads

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

protein G

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 28-9670-70

storage condition

(20% Ethanol, 10% medium slurry)

parameter

Room temperature temp. range

matrix

paramagnetic, spherical, highly cross-linked agarose particles

capacity

27 mg binding capacity(human IgG/ml gel)

storage temp.

2-8°C

Related Categories

General description

protein G Mag Sepharose Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG /mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Application

Protein G Mag Sepharose Xtra magnetic beads combine proven affinity chromatography methods with the magnetic bead platform to improve simplification and ease-of-use in sample preparation. Mag Sepharose beads are dense, black and visible, and this makes them simple to handle. Less smearing effects and aggregate formations of the magnetic beads mean that no detergents are needed in the buffers.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.


Protein G Mag Sepharose is another product in the Mag Sepharose platform which is designed for the rapid capture and enrichment of Protein samples from cell lysates and biological fluids using immunoprecipitation technology. Protein G Mag Sepharose is made with an optimized ligand for immunoprecipitation.

Features and Benefits

  • Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
  • High purity and yield
  • Robust parallel screening of antibodies with high reproducibility
  • Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
  • Visible and dense Sepharose based magnetic beads give increased simplicity for handling
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Flame

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Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

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Articles

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service