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Safety Information

GE28-9645-13

Sigma-Aldrich

HiScale 26/40

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UNSPSC Code:
12352200
NACRES:
SB.52

technique(s)

LPLC: suitable

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General description

The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments as well as disassembly and cleaning. The measurement scale on the column makes it easier to predict and reach a predefined packing height, thereby increasing process control as well as reproducibility. Although optimized for high-pressure media, HiScale columns are fully compatible with all applications (and packing methods) currently applicable to XK columns.

Features and Benefits

Adapter QuickLock mechanism, column measurement scale, and ergonomic design, facilitate column handling and increase process control.
Based on the well-established line of XK columns and compatible with all methods currently applicable to XK columns.
Capability to rinse entire adapter shaft after packing, thereby minimizing risk of bacterial growth after prolonged use.
Dual adapters, offering high flexibility and dynamic packing heights.
Empty columns with high pressure stability for packing with modern BioProcess media for process development and preparative chromatography.
Provides axial compression of the gel bed and a nonrotating plunger mechanism that expands packing capabilities.

Regulatory Information

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Articles

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

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This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Column Packing and Preparation for Size Exclusion Chromatography

Column Packing and Preparation for Size Exclusion Chromatography

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