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Merck
CN

GE28-9644-41

HiScale 16/20

Synonym(s):

HiScale Column

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About This Item

NACRES:
SB.52
UNSPSC Code:
12352200

Key Documents

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technique(s)

LPLC: suitable

Features and Benefits

Adapter QuickLock mechanism, column measurement scale, and ergonomic design, facilitate column handling and increase process control.
Based on the well-established line of XK columns and compatible with all methods currently applicable to XK columns.
Capability to rinse entire adapter shaft after packing, thereby minimizing risk of bacterial growth after prolonged use.
Dual adapters, offering high flexibility and dynamic packing heights.
Empty columns with high pressure stability for packing with modern BioProcess media for process development and preparative chromatography.
Provides axial compression of the gel bed and a nonrotating plunger mechanism that expands packing capabilities.

General description

HiScale columns are delivered with a column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors.
HiScale columns are designed to withstand pressures of up to 20 bar (290 psi) and high flow rates, making them compatible with modern BioProcess chromatography media such as MabSelect and Capto. The user-friendly design allows for an optimized process, through simplified and reproducible operation.

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Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

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Protocols

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Column Packing and Preparation for Size Exclusion Chromatography

Column Packing and Preparation for Size Exclusion Chromatography

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

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