GE28-9574-99
TALON® Superflow™
Cytiva 28-9574-99, pack of 10 mL
Synonym(s):
TALON Affinity Resin
About This Item
ligand
TALON® Superflow Cobalt-IMAC
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 28-9574-99
matrix
6% cross-linked agarose
particle size
60-160 μm
cleaning in place
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
capacity
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.
2-8°C
Related Categories
General description
Features and Benefits
- Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protocols
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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