GE28-9537-66
HiTrap® TALON® Crude
Cytiva 28-9537-66, pack of 5 × 1 mL
Recommended Products
ligand
TALON® Superflow Cobalt-IMAC
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 28-9537-66
parameter
1 ml/min - 4 ml/min flow rate
3 bar
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
60-160 μm
cleaning
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
capacity
≥20 mg binding capacity (histidine-tagged protein)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.
2-8°C
Related Categories
General description
Application
HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.
Features and Benefits
- Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of Proteins.
- HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.
Analysis Note
Other Notes
Legal Information
Signal Word
Warning-Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
100.4 - 109.4 °F
Flash Point(C)
38 - 43 °C
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protocols
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
How to isolate proteins from inclusion bodies using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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