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ligand
glutathione
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pkg of 10 ea
manufacturer/tradename
Cytiva 28-9523-60
bed volume
2 mL
column I.D.
15 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-13
capacity
≥20 mg binding capacity(recombinant glutathione S-transferase (rGST))
>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)
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General description
Application
Features and Benefits
- Convenient, prepacked gravity-fLow columns.
- Allows direct purification of both clarified and unclarified cell lysates.
- Yields up to 20 mg of pure Proteins per column.
- Yields up to 20 mg of pure Proteins per column
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protocols
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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