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GE28-9466-03

HiTrap® Albumin IgG Depletion

Cytiva 28-9466-03, pack of 2 × 1 mL

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UNSPSC Code:
41105323
NACRES:
NA.56

ligand

recombinant protein G fragment and recombinant protein binding HSA

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

wetted part: no

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 28-9466-03

storage condition

(20% Ethanol)

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

avg. part. size

34 μm

cleaning

2-9

working range

3-9

storage temp.

2-8°C

General description

HiTrap® Albumin & IgG Depletion is prepacked columns for the depletion of albumin and IgG from human serum and plasma. The columns are prepacked with high performance SepharoseTM based media with an affinity for human serum albumin (HSA) and IgG.

Application

HiTrap® Albumin & IgG Depletion and Albumin & IgG Depletion SpinTrap™ are prepacked columns for the depletion of albumin and IgG from plasma and serum samples. Albumin and IgG are the most abundant plasma Proteins, and they tend to obscure the signals of less abundant Proteins. Depletion of the two Proteins removes more than 60% of the total Protein content in human plasma, allowing a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins. These columns are members of the Trap platform and address the need for flexible, small-scale preparation of Protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.

Features and Benefits

  • HiTrap® Albumin & IgG Depletion is a prepacked column for the depletion of albumin and IgG from human serum or plasma.
  • HiTrap® Albumin & IgG Depletion column provides rapid and easy processing in a convenient format for handling larger sample volumes (~150 μL) using a liquid chromatography system or manually using a syringe.
  • Columns are prepacked with high performance Sepharose™ based media with an affinity for human serum albumin (HSA) and IgG.
  • High depletion capacity, removes > 95% HSA and > 90% IgG, with high reproducibility.
  • Simple and fast procedure.
  • Removal of albumin and IgG allows a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Regulatory Information

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Certificates of Analysis (COA)

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Protocols

Purification or Removal of Albumin and IgG

This page shows how to purify or remove albumin and IgG with Albumin & IgG Depletion Sepharose High Performance from Cytiva.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service