GE28-9365-50
GSTPrep™ Fast Flow 16/10
Cytiva 28-9365-50
Recommended Products
ligand
glutathione and 10-carbon linker arm
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pkg of 20 mL
manufacturer/tradename
Cytiva 28-9365-50
parameter
<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
3-12
working range
3-12
capacity
>10 mg/mL binding capacity(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
Related Categories
General description
Application
Features and Benefits
- Fast and easy one-step, scale-up purification of GST-tagged Proteins.
- Binds approx. 200 mg recombinant GST/column
- Mild elution conditions preserving Protein antigenicity and function.
- Simple operation with a pump or chromatography system, such as ÄKTA® design.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
Protocols
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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