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GE28-9365-40

HiPrep SP XL 16/10

Cytiva 28-9365-40

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UNSPSC Code:
47101511
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 20 mL

manufacturer/tradename

Cytiva 28-9365-40

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiPrep SP XL 16/10 is a strong cation exchanger prepacked with Q Sepharose XL columns for inital capture of proteins and other biomoleculs with ion exchange (IEX) chromatography.

Application

HiPrep SP XL 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provides fast, preparative separations of Proteins and other biomolecules.

Features and Benefits

  • Provide high loading capacity and fast purifications.
  • Optimized for reliable, reproducible separations.
  • Compatible with simple pump-based configurations and chromatography systems such as ÄKTA® design.
  • Excellent choice for rapid enrichment during initial capture of Proteins from start material.
  • Strong Sulfopropyl (SP) cation exchanger.

Storage and Stability

Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

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Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page covers how and when to use Sepharose XL media for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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