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GE28-9356-00

StrepTactin Sepharose High Performance

Cytiva 28-9356-00, pack of 50 mL

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UNSPSC Code:
41106500
NACRES:
NA.56

ligand

StrepTactin

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 50 mL

manufacturer/tradename

Cytiva 28-9356-00

storage condition

(20% Ehtanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

working range

7

capacity

~6 mg binding capacity (Strep II-tagged protein/ml medium)(Binding capacity is protein dependent. Dynamic binding capacity was tested with GAPDH-Strep(II), Mr 37 400.)

shipped in

wet ice

storage temp.

2-8°C

General description

StrepTactin Sepharose High Performance media for robust, high resolution purification of Strep II-tagged proteins. Mild purification conditions preserve antigenicity and function.

Application

This robust and stable Sepharose based medium results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also good.

Features and Benefits

  • Highly pure Strep II-tagged recombinant Proteins eluted in concentrated form and small volumes
  • Physiological conditions and mild elution preserve target Protein activity
  • Fast and easy regeneration with 0.5 M NaOH
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Certificates of Analysis (COA)

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Protocols

This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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