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GE28-9355-99

Streptactin Sepharose™ High Performance

Cytiva 28-9355-99, pack of 10 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

StrepTactin

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 28-9355-99

storage condition

(20% Ehtanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

working range

7

capacity

~6 mg binding capacity (Strep II-tagged protein/ml medium)

shipped in

wet ice

storage temp.

2-8°C

General description

Binding capacity is protein dependent. Dynamic binding capacity was tested with GAPDH-Strep(II), Mr 37 400.
StrepTactin Sepharose™ High Performance media for robust, high resolution purification of Strep II-tagged proteins. Mild purification conditions preserve antigenicity and function. This robust and stable Sepharose™ based medium results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also good.

Features and Benefits

  • Highly pure Strep II-tagged recombinant proteins eluted in concentrated form and small volumes
  • Physiological conditions and mild elution preserve target protein activity
  • Fast and easy regeneration with 0.5 M NaOH
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

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Protocols

Purification using StrepTactin Sepharose® High Performance

This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.

Purification using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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