GE28-9355-98
Dextrin Sepharose™ High Performance
Cytiva 28-9355-98, pack of 100 mL
Recommended Products
ligand
dextrin
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 28-9355-98
storage condition
(20% Ehtanol)
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning in place
2-13
working range
>7
capacity
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)(Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).)
storage temp.
2-8°C
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General description
Application
Features and Benefits
- Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
- The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
- Physiological conditions and mild elution preserve target Protein activity
- Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications
Analysis Note
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Certificates of Analysis (COA)
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Articles
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Protocols
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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