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GE28-9075-46

Sigma-Aldrich

StrepTrap™ High Performance prepacked chromatography column

pack of 5 × 1 mL, prepacked chromatography column

Synonym(s):

StrepTrap™ High Performance

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

StrepTactin

grade

for chromatography

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 28-9075-46

storage condition

(20% Ehtanol)
 (20% Ethanol)

parameter

<4 mL/min flow rate
42 psi (H2O at room temperature.)

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

working range

7

capacity

~6 mg binding capacity (Strep II-tagged protein/ml medium)

storage temp.

2-8°C

General description

StrepTrap HP is a ready to use HiTrap® column prepacked with StrepTactin Sepharose™ High Performance for purifying Strep-tag II recombinant proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

Tagged purification using the Strep-tag.

Features and Benefits

  • Highly pure Strep-tag II recombinant proteins eluted in concentrated form and small volumes.
  • Physiological conditions and mild elution preserves the activity of the target protein.
  • Convenient, time-saving operation and reproducible results.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
StrepTrap is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

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Articles

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service