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GE28-9034-08

ProbeQuant G-50 Micro Columns

Cytiva 28-9034-08, pack of 50 columns

Synonym(s):

Micro Columns

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

packaging

pack of 50 columns

manufacturer/tradename

Cytiva 28-9034-08

particle size

50-150 μm (dry)

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General description

ProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides.

ProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex® G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μ to 50 μ.

Features and Benefits

  • For rapid purification of labeled DNA (20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography
  • Convenient: Prepacked with Sephadex® G-50 DNA Grade (see illustra Sephadex® G-50 and G-100 DNA Grade) and pre-equilibrated in STE containing 0.15% Kathon CG/ICP Biocide
  • Ready to use: Requires less than 4 min from sample application to collection of purified product
  • Designed for use in a microcentrifuge
  • Tested in nickase, single and double-stranded exonuclease and RNase assays

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
illustra is a trademark of Cytiva

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Caroline M Peres et al.
Journal of bacteriology, 188(24), 8662-8665 (2006-10-17)
The rate-limiting enzyme of anaerobic benzoate degradation by Rhodopseudomonas palustris, benzoyl coenzyme A (CoA) reductase, is highly sensitive to oxygen, and its synthesis is tightly regulated. We determined that a previously unknown gene in the benzoate gene cluster, badM, encodes
R J Akhurst et al.
International journal of systematic and evolutionary microbiology, 54(Pt 4), 1301-1310 (2004-07-29)
The relationship of Photorhabdus isolates that were cultured from human clinical specimens in Australia to Photorhabdus asymbiotica isolates from human clinical specimens in the USA and to species of the genus Photorhabdus that are associated symbiotically with entomopathogenic nematodes was
Bogac Kaynak et al.
Circulation, 107(19), 2467-2474 (2003-05-14)
We present the first genome-wide cDNA array analysis of human congenitally malformed hearts and attempted to partially elucidate these complex phenotypes. Most congenital heart defects, which account for the largest number of birth defects in humans, represent complex genetic disorders.
K Higuchi et al.
Plant physiology, 119(2), 471-480 (1999-02-10)
Nicotianamine synthase (NAS), the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores, catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine. We purified NAS protein and isolated the genes nas1, nas2, nas3, nas4
T Wada et al.
Journal of the American Society of Nephrology : JASN, 12(12), 2601-2608 (2001-12-01)
Mesangial cells play an important role in maintaining glomeruli structure and function and in the pathogenesis of glomerular diseases. With a novel approach using a rapid large-scale DNA sequencing strategy and computerized data processing, a new human gene, PP4(Rmeg) was

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