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Merck
CN

GE28-4082-60

HiTrap® MabSelect™ Xtra

Cytiva 28-4082-60, pack of 1 × 5 mL

Synonym(s):

affinity column, chromatography column, mAb purification column, prepacked column, protein A column

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816

Key Documents

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Product Name

HiTrap® MabSelect™ Xtra, Cytiva 28-4082-60, pack of 1 × 5 mL

ligand

recombinant protein A (E. coli)

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 28-4082-60

storage condition

(20% Ehtanol)
 (20% Ethanol)

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked agarose

avg. part. size

75 (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

working range

3-10

capacity

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

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Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect medium.
  • MabSelect Xtra uses the same recombinant protein A ligand as MabSelect
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

General description

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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