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GE28-4064-08

Sigma-Aldrich

Tricorn 5/20 Column

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About This Item

UNSPSC Code:
41121800
NACRES:
SB.52

technique(s)

LPLC: suitable

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General description

Tricorn Packing Equipment includes the Packing Equipment 5/50 (5-mm connector and 50-mm glass tube) or 10/100 (10-mm connector and 100-mm glass tube), EPDM O-rings and bottom unit, cap, and stop plug.
Tricorn columns include a glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock, and filter holder.

Features and Benefits

Broad range of column dimensions suitable for many different purification needs.
Glass tubes coated with plastic film prevent harm if the column is dropped or operated outside its specification.
Locking ring prevents accidental compression of media bed.
Separate packing connector allows simple column packing for a broad range of media.
The modern integrated, snap-on design results in a column that is easy to assembly, operate, and maintain.
Tricorn empty columns are designed for high performance separations on small and medium scale. Pressure stability up to 100 bar and durable column material ensure reliable and reproducible operation as well as compatibility with the majority of the commercial lab media.

Regulatory Information

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Certificates of Analysis (COA)

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Articles

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Converting from Flow Velocity to Volumetric Flow Rates

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Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

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Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

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Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

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Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

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