GE28-4013-53
His Spintrap™
Cytiva 28-4013-53
Synonym(s):
His Spintrap Column
About This Item
manufacturer/tradename
Cytiva 28-4013-53
packaging
pkg of 50 x 100 μL
bed volume
100 μL
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
Related Categories
General description
* See the section for licensing information in our terms and conditions.
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap™ together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose™ High Performance medium is negligible.
Features and Benefits
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose™ High Performance in prepacked spin columns.
- Yields up to 750 μg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
12 - Non Combustible Liquids
Regulatory Information
Certificates of Analysis (COA)
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Articles
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protocols
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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